0000026934 00000 n 0000020645 00000 n %%EOF 0000004800 00000 n 0000004465 00000 n 0000064427 00000 n 2 Genome Biology Vol 3 No 12 Tomancak et al. 0000010032 00000 n We describe high-throughput in situ hybridization in 96-well plates as example application for the mass-isolated egg chambers. Isolation of egg chambers from adult flies can be completed in 5 min and results, depending on the input amount of flies, in several milliliters of material.
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0000064219 00000 n (3)Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany. 0000007209 00000 n 0000013568 00000 n Springer Harrington, Pavel Tomancak, Stefan Gumhold, Ivo F. Sbalzarini. Name must be less than 100 characters 0000084721 00000 n 0000042810 00000 n
��'s�Dy�������HT��K��i]_�6��n��JF����\>��\۽1j�������c���u�Z�ϩ'�y��f%W���ƙWw�xxt�U�4� website. 0000006895 00000 n 0000002281 00000 n 0000008166 00000 n 0000065074 00000 n 0000007053 00000 n Two methods have been used successfully to determine gene-expression patterns on a large scale - RNA in situ hybridization [1] and DNA microarrays [2-4]. T. Kalinka and Pavel Tomancak Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 0000006577 00000 n
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Springer 0000026713 00000 n (2)Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany unst@bfr.bund.de tomancak@mpi-cbg.de. 0000084093 00000 n 0 0000083417 00000 n 0000084230 00000 n 0000084586 00000 n segs' and drugs - segmental phenotypes in zebrafish Modular small molecule screening identifies novel vertebrate segmentation defects Sandra Richter 1,2,4, Ulrike Schulze 2,4, Pavel Tomancak 1 and Andrew C.Oates 1,2,3,4 1 Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. 0000003589 00000 n
0000026057 00000 n Pavel Tomancak (MPI-CBG) Patterns of gene expression in animal development. 0000026541 00000 n trailer
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Ulrik Günther, Tobias Pietzsch, Aryaman Gupta, Kyle I.S. The isolated egg chambers are then further processed depending on the exact requirements of the subsequent application. This site needs JavaScript to work properly. 0000036789 00000 n <<88826696DF63834E9B114545101787DC>]/Prev 832209/XRefStm 3073>> 0000006018 00000 n We work in close collaboration with biologists in the Zerial Lab at the MPI-CBG and theorists at the MPI-PKS to perform large-scale analytics on the tissue such as statistics on the cell localization, size, polarity, density, and connectivity, to aid the formulation of multi-scale models for systems biology. 2015;1328:137-49. doi: 10.1007/978-1-4939-2851-4_10.Methods Mol Biol.
0000003507 00000 n 0000006418 00000 n 0000063930 00000 n Please enable it to take advantage of the complete set of features! Régis Lemaitre. 0000046790 00000 n Pavel Tomancak Group Leader at MPI-CBG Dresden und Umgebung, Deutschland 457 Kontakte. Zum Vernetzen anmelden. Animal embryos shape their tissues during development through a variety of mechanisms, one of which involves coordinated constriction of one side of a sheet of cells, leading to tissue bending. 0000006258 00000 n 0000006656 00000 n 0000009323 00000 n Isolation of large quantities of tissue from organisms is essential for many techniques such as genome-wide screens and biochemistry. The isolated egg chambers are amenable for a variety of procedures such as fluorescent in situ hybridization, RNA isolation, extract preparation, or immunostaining. On page 326 of this issue, Brunet et al. 0000034359 00000 n 0000004912 00000 n Christoph Weber (MPI-PKS) Mesoscopic Physics of Live. startxref This apical constriction mechanism of tissue morphogenesis occurs in most animal groups ([ 1 ][1]), suggesting that it was inherited from a common ancestor. 0000041418 00000 n 0000009808 00000 n
0000030066 00000 n 0000007131 00000 n 0000038891 00000 n Dunst S(1), Tomancak P(2). 0000025174 00000 n 2016;1478:215-226. doi: 10.1007/978-1-4939-6371-3_12.Methods Mol Biol. 0000003073 00000 n Christoph Zechner (MPI-CBG) Adaptive Biosystems. 0000006338 00000 n
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... Vanessa de Oliveira Carlos ... Research Group Leader Biophysics at MPI-CBG - Max Planck Institute of Molecular Cell Biology and Genetics. 369 0 obj <> endobj 0000005473 00000 n Pavel Tomancak , Dr. 3 Emmanuel Reynaud , Dr. 4 1 Leica Microsystems 2 IGBMC - Institute of Genetics and Molecular and Cellular Biology, France 3 Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany 0000004688 00000 n 0000006974 00000 n 0000084394 00000 n 0000006736 00000 n
0000025722 00000 n Clipboard, Search History, and several other advanced features are temporarily unavailable. 3 Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Wako City, Saitama 351-0198, Japan. 0000000016 00000 n Epub 2016 Jan 28. website. 0000008861 00000 n 0000024943 00000 n 0000027217 00000 n 0000083674 00000 n 0000003246 00000 n Methods Mol Biol.
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0000014075 00000 n 0000083456 00000 n 0000055017 00000 n 0000003625 00000 n 0000004130 00000 n 0000042458 00000 n Isolation of large quantities of tissue from organisms is essential for many techniques such as genome-wide screens and biochemistry.